#scrnaseq search results

Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu

mustafa_soytas's tweet image. Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu
mustafa_soytas's tweet image. Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu
mustafa_soytas's tweet image. Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu

Neuronal diversity is written in transcriptional codes 🧬. But what is the logic of these codes that define cell types and wiring patterns? To find out we built a #scRNAseq developmental atlas of the Drosophila nerve cord and linked it to the #connectome 🪰🧠 Tweeprint! ⬇️1/8

gsxej's tweet image. Neuronal diversity is written in transcriptional codes 🧬. But what is the logic of these codes that define cell types and wiring patterns?
To find out we built a #scRNAseq developmental atlas of the Drosophila nerve cord and linked it to the #connectome 🪰🧠
Tweeprint! ⬇️1/8

TemporalVAE: atlas-assisted temporal mapping of time-series single-cell transcriptomes during embryogenesis. #SingleCell #scRNAseq #TimeSeries #Embryogenesis #DeepLearning #Genomics #Bioinformatics @NatureCellBio nature.com/articles/s4155…

jsantoyo's tweet image. TemporalVAE: atlas-assisted temporal mapping of time-series single-cell transcriptomes during embryogenesis. #SingleCell #scRNAseq #TimeSeries #Embryogenesis #DeepLearning #Genomics #Bioinformatics @NatureCellBio  
nature.com/articles/s4155…

Our newest preprint is out! We introduce Forseti, a model to predict the splicing status of individual fragments in tagged-end #scRNAseq experiments (biorxiv.org/content/10.110…). 1/

nomad421's tweet image. Our newest preprint is out! We introduce Forseti, a model to predict the splicing status of individual fragments in tagged-end #scRNAseq experiments (biorxiv.org/content/10.110…). 1/

🚨 New preprint alert! 🚨 We used single-cell RNA-seq + GRN analysis to uncover novel morphogenic genes that boost #maize transformation frequency 🌽 biorxiv.org/content/10.110… Thanks to @plaza_genomics and all other co-authors! #PlantScience #scRNAseq

LaurensPauwels's tweet image. 🚨 New preprint alert! 🚨

We used single-cell RNA-seq + GRN analysis to uncover novel morphogenic genes that boost #maize transformation frequency 🌽

biorxiv.org/content/10.110…

Thanks to @plaza_genomics and all other co-authors!

#PlantScience #scRNAseq

#AI methods for #denoising of #scRNAseq data have recently raised huge attention Yet, existing approaches lead to dramatically different (dangerous?) data transformations In the most extensive review to date we compare 19 methods and provide guidelines doi.org/10.1093/bib/bb…

AGraudenzi's tweet image. #AI methods for #denoising of #scRNAseq data have recently raised huge attention
Yet, existing approaches lead to dramatically different (dangerous?) data transformations

In the most extensive review to date we compare 19 methods and provide guidelines
doi.org/10.1093/bib/bb…

Had a great time giving my education talk on decoding preclinical models of neuropathies using #scRNAseq 🧬 🧫🔬 @PNSociety1 Really appreciated the insightful questions and engaging discussion!

EidSteph's tweet image. Had a great time giving my education talk on decoding preclinical models of neuropathies using #scRNAseq 🧬 🧫🔬 @PNSociety1 

Really appreciated the insightful questions and engaging discussion!
EidSteph's tweet image. Had a great time giving my education talk on decoding preclinical models of neuropathies using #scRNAseq 🧬 🧫🔬 @PNSociety1 

Really appreciated the insightful questions and engaging discussion!

New blog post! How to make a multi-group dotplot for single-cell RNAseq data from scratch buff.ly/3SmHeEm #rstats #scRNAseq

tangming2005's tweet image. New blog post! How to make a multi-group dotplot for single-cell RNAseq data from scratch buff.ly/3SmHeEm #rstats #scRNAseq

Benchmarking scRNA-seq copy number variation callers. #scRNAseq #CNV #Benchmarking @NatureComms nature.com/articles/s4146…

jsantoyo's tweet image. Benchmarking scRNA-seq copy number variation callers. #scRNAseq #CNV #Benchmarking @NatureComms  
nature.com/articles/s4146…

1/n Just in time for the Holidays "Predicting lung aging using #scRNASeq data" is out!! #NormalLungAgingCellAtlas team report sa novel method that predicts lung age in smokers and non-smokers. Kudos lead authors, Drs Song, Bar-Joesph & team. See 🧵below doi.org/10.1371/journa…

KaminskiMed's tweet image. 1/n
Just in time for the Holidays "Predicting lung aging using #scRNASeq data" is out!! #NormalLungAgingCellAtlas team report sa novel method that predicts lung age in smokers and non-smokers. Kudos lead authors, Drs Song, Bar-Joesph &  team. See 🧵below 
doi.org/10.1371/journa…

MitoDelta: identifying mitochondrial DNA deletions at cell-type resolution from single-cell RNA sequencing data. #mtDNA #SingleCell #scRNAseq #Sequencing #Genomics #Bioinformatics #BMCgenomics bmcgenomics.biomedcentral.com/articles/10.11…

jsantoyo's tweet image. MitoDelta: identifying mitochondrial DNA deletions at cell-type resolution from single-cell RNA sequencing data. #mtDNA #SingleCell #scRNAseq #Sequencing #Genomics #Bioinformatics #BMCgenomics 
bmcgenomics.biomedcentral.com/articles/10.11…

Lots of applications of AI for #scRNAseq analysis developed in the ⁦@vafaee_fatemeh⁩ lab, including SAFAARI for cell type annotation #AGTA24

mritchieau's tweet image. Lots of applications of AI for #scRNAseq analysis developed in the ⁦@vafaee_fatemeh⁩ lab, including SAFAARI for cell type annotation  #AGTA24

One of the curiosities of genomics history is that James Watson was vehemently against cDNA sequencing (for ESTs), and fought with Craig Venter & Bernadine Healy who championed it. Tl;dr Watson ended up resigning from the HGP, Venter plowed ahead... and we now have #scRNAseq.


Check out our Spotlight article (w/ @JoeEcker), featuring a recent paper on plant responses to bacterial pathogens using #scRNAseq. We also share our thoughts on how #singlecell and #spatial omics technologies could address unanswered questions in #MPMI. doi.org/10.1016/j.crme…

nobolly's tweet image. Check out our Spotlight article (w/ @JoeEcker), featuring a recent paper on plant responses to bacterial pathogens using #scRNAseq. We also share our thoughts on how #singlecell and #spatial omics technologies could address unanswered questions in #MPMI.
doi.org/10.1016/j.crme…

🚀Meet the 𝐀𝐭𝐥𝐚𝐬 𝐨𝐟 𝐍𝐞𝐮𝐭𝐫𝐨𝐩𝐡𝐢𝐥 𝐁𝐢𝐨𝐥𝐨𝐠𝐲 & its companion app 𝑁𝑒𝑢𝑡𝑟𝑜𝑝ℎ𝑖𝑙 𝐶𝑎𝑛𝑣𝑎𝑠. — a hub for exploring #neutrophils with #scRNAseq. Discover, compare & interact with #singlecell neutrophil across studies. #Bioinformatics #DataViz #OpenScience

fengjun_zhang's tweet image. 🚀Meet the 𝐀𝐭𝐥𝐚𝐬 𝐨𝐟 𝐍𝐞𝐮𝐭𝐫𝐨𝐩𝐡𝐢𝐥 𝐁𝐢𝐨𝐥𝐨𝐠𝐲 & its companion app 𝑁𝑒𝑢𝑡𝑟𝑜𝑝ℎ𝑖𝑙 𝐶𝑎𝑛𝑣𝑎𝑠. — a hub for exploring #neutrophils with #scRNAseq. Discover, compare & interact with #singlecell neutrophil across studies. #Bioinformatics #DataViz #OpenScience

BestopCloud: an integrated one-stop solution for single-cell RNA sequencing data analysis. #SingleCell #scRNAseq #DataAnalysis #WebTool #OpenSource #Rshiny #BMCgenomics bmcgenomics.biomedcentral.com/articles/10.11…

jsantoyo's tweet image. BestopCloud: an integrated one-stop solution for single-cell RNA sequencing data analysis. #SingleCell #scRNAseq #DataAnalysis #WebTool #OpenSource #Rshiny #BMCgenomics
bmcgenomics.biomedcentral.com/articles/10.11…

ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells. #SingleCell #scRNAseq #Isoforms @GenomeBiology genomebiology.biomedcentral.com/articles/10.11…

jsantoyo's tweet image. ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells. #SingleCell #scRNAseq #Isoforms @GenomeBiology
genomebiology.biomedcentral.com/articles/10.11…
jsantoyo's tweet image. ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells. #SingleCell #scRNAseq #Isoforms @GenomeBiology
genomebiology.biomedcentral.com/articles/10.11…

Discover the future of scRNA sequencing! Dr. Simone Picelli discusses seqWell's plexWell™ Rapid Single Cell kit—high sensitivity, faster workflow & miniaturization potential. Watch the webinar: hubs.li/Q03y-X1x0 #Webinar #scRNAseq #SingleCell

sambasci's tweet card. Resolution at Scale Plexwell Rapid Single Cell for scRNA Sequencing -...

youtube.com

YouTube

Resolution at Scale Plexwell Rapid Single Cell for scRNA Sequencing -...


One of the curiosities of genomics history is that James Watson was vehemently against cDNA sequencing (for ESTs), and fought with Craig Venter & Bernadine Healy who championed it. Tl;dr Watson ended up resigning from the HGP, Venter plowed ahead... and we now have #scRNAseq.


Single-cell RNA sequencing reveals developmental trajectories and environmental regulation of callus formation in Arabidopsis Zhixin Liu, Yixin Zhang ... Guoyong An, Xuwu Sun link.springer.com/article/10.100… #ScRNAseq #regeneration #dedifferentiation


#Article in @MedComm_MC Discovering Heterogeneous Leukocytes Subsets Associated With Alcoholic Steatohepatitis by #scRNAseq Analysis doi.org/10.1002/mco2.7… #AlcoholicLiverDisease #AlcoholicSteatohepatitis #Bcell #ImmuneCell #Neutrophil #tSNEs

MedComm_MC's tweet image. #Article in @MedComm_MC 
Discovering Heterogeneous Leukocytes Subsets Associated With Alcoholic Steatohepatitis by #scRNAseq Analysis doi.org/10.1002/mco2.7…

#AlcoholicLiverDisease #AlcoholicSteatohepatitis #Bcell #ImmuneCell #Neutrophil #tSNEs

Join our 3-day course to learn how to design, generate, and analyse #scRNAseq data — from theory to hands-on practice with droplet-based platforms. Perfect for anyone exploring gene expression at single-cell resolution. Registration: elixir-czech.cz/registration/e…

ELIXIRCZ's tweet image. Join our 3-day course to learn how to design, generate, and analyse #scRNAseq data — from theory to hands-on practice with droplet-based platforms.
Perfect for anyone exploring gene expression at single-cell resolution. Registration: elixir-czech.cz/registration/e…

Master Single-cell RNA-seq data analysis! 🧬 Register here ---> lnkd.in/euaapbNq Join TIBR biotech for a 5-day hands-on virtual workshop from November 13-18, 2025. Perfect for students, researchers, and aspiring bioinformaticians. #scRNAseq #Bioinformatics #DataAnalysis

DrNobleKKurian's tweet image. Master Single-cell RNA-seq data analysis! 🧬
Register here ---> lnkd.in/euaapbNq
Join TIBR biotech for a 5-day hands-on virtual workshop from November 13-18, 2025.
Perfect for students, researchers, and aspiring bioinformaticians.
#scRNAseq #Bioinformatics #DataAnalysis

Unveiled single-cell RNA & spatial metabolomics in Panax root tips, revealing gene patterns & ginsenosides biosynthesis. #PanaxGenomics #scRNAseq @OxfordJournals Details: doi.org/10.1093/hr/uha…

Hortres's tweet image. Unveiled single-cell RNA & spatial metabolomics in Panax root tips, revealing gene patterns & ginsenosides biosynthesis. #PanaxGenomics #scRNAseq @OxfordJournals
Details: doi.org/10.1093/hr/uha…

Discover the applications for single-cell analysis & learn about the common challenges and their resolutions in our whitepaper. View it now! ⬇️ fiosgenomics.com/wp-content/upl… #singlecell #scRNAseq #bioinformatics


Discover how analysis of scRNA-seq data supports AAV gene therapy research. You will learn how it helps with everything from identifying target cells to predicting treatment response and resistance! fiosgenomics.com/advancing-aav-… #genetherapy #scRNAseq #bioinformatics


Master Single-cell RNA-seq data analysis! 🧬 Register here ---> lnkd.in/euaapbNq Join TIBR biotech for a 5-day hands-on virtual workshop from November 13-18, 2025. Perfect for students, researchers, and aspiring bioinformaticians. #scRNAseq #Bioinformatics #TIBRBiotech

DrNobleKKurian's tweet image. Master Single-cell RNA-seq data analysis! 🧬
Register here ---> lnkd.in/euaapbNq
Join TIBR biotech for a 5-day hands-on virtual workshop from November 13-18, 2025.
Perfect for students, researchers, and aspiring bioinformaticians.
#scRNAseq #Bioinformatics  #TIBRBiotech

Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu

mustafa_soytas's tweet image. Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu
mustafa_soytas's tweet image. Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu
mustafa_soytas's tweet image. Proud to be a researcher of this great kidney cancer genome project efforts for more than 2 years will impact the future of #RenalCellCarcinoma #CancerResearch #scRNAseq is presented #AUA24 @AmerUrological @SiTanguay @YRiazalhosseini @fadbrimo @McGillGenome @RIMUHC1 @mcgillu

Neuronal diversity is written in transcriptional codes 🧬. But what is the logic of these codes that define cell types and wiring patterns? To find out we built a #scRNAseq developmental atlas of the Drosophila nerve cord and linked it to the #connectome 🪰🧠 Tweeprint! ⬇️1/8

gsxej's tweet image. Neuronal diversity is written in transcriptional codes 🧬. But what is the logic of these codes that define cell types and wiring patterns?
To find out we built a #scRNAseq developmental atlas of the Drosophila nerve cord and linked it to the #connectome 🪰🧠
Tweeprint! ⬇️1/8

I do rather like this one. #scRNAseq

erikacule's tweet image. I do rather like this one. #scRNAseq

Check out our Spotlight article (w/ @JoeEcker), featuring a recent paper on plant responses to bacterial pathogens using #scRNAseq. We also share our thoughts on how #singlecell and #spatial omics technologies could address unanswered questions in #MPMI. doi.org/10.1016/j.crme…

nobolly's tweet image. Check out our Spotlight article (w/ @JoeEcker), featuring a recent paper on plant responses to bacterial pathogens using #scRNAseq. We also share our thoughts on how #singlecell and #spatial omics technologies could address unanswered questions in #MPMI.
doi.org/10.1016/j.crme…

Benchmarking scRNA-seq copy number variation callers. #scRNAseq #CNV #Benchmarking @NatureComms nature.com/articles/s4146…

jsantoyo's tweet image. Benchmarking scRNA-seq copy number variation callers. #scRNAseq #CNV #Benchmarking @NatureComms  
nature.com/articles/s4146…

Candidate drug targets with #scRNAseq support defined as 👉cell type specific expression in disease-relevant tissue OR 👉cell type specific over-expression in disease patients are more likely to successfully advance from research to clinic medrxiv.org/content/10.110…

MariosGeorgakis's tweet image. Candidate drug targets with #scRNAseq support defined as

👉cell type specific expression in disease-relevant tissue 
OR 
👉cell type specific over-expression in disease patients 

are more likely to successfully advance from research to clinic

medrxiv.org/content/10.110…
MariosGeorgakis's tweet image. Candidate drug targets with #scRNAseq support defined as

👉cell type specific expression in disease-relevant tissue 
OR 
👉cell type specific over-expression in disease patients 

are more likely to successfully advance from research to clinic

medrxiv.org/content/10.110…

ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells. #SingleCell #scRNAseq #Isoforms @GenomeBiology genomebiology.biomedcentral.com/articles/10.11…

jsantoyo's tweet image. ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells. #SingleCell #scRNAseq #Isoforms @GenomeBiology
genomebiology.biomedcentral.com/articles/10.11…
jsantoyo's tweet image. ScIsoX: a multidimensional framework for measuring isoform-level transcriptomic complexity in single cells. #SingleCell #scRNAseq #Isoforms @GenomeBiology
genomebiology.biomedcentral.com/articles/10.11…

shinyUMAP: an online tool for promoting understanding of single cell omics data visualization. #SingleCell #scRNAseq #DataVisualization #Genomics #Bioinformatics @biorxiv_bioinfo biorxiv.org/content/10.110…

jsantoyo's tweet image. shinyUMAP: an online tool for promoting understanding of single cell omics data visualization. #SingleCell #scRNAseq #DataVisualization #Genomics #Bioinformatics @biorxiv_bioinfo 
biorxiv.org/content/10.110…

🚨 New preprint alert! 🚨 We used single-cell RNA-seq + GRN analysis to uncover novel morphogenic genes that boost #maize transformation frequency 🌽 biorxiv.org/content/10.110… Thanks to @plaza_genomics and all other co-authors! #PlantScience #scRNAseq

LaurensPauwels's tweet image. 🚨 New preprint alert! 🚨

We used single-cell RNA-seq + GRN analysis to uncover novel morphogenic genes that boost #maize transformation frequency 🌽

biorxiv.org/content/10.110…

Thanks to @plaza_genomics and all other co-authors!

#PlantScience #scRNAseq

New blog post! How to make a multi-group dotplot for single-cell RNAseq data from scratch buff.ly/3SmHeEm #rstats #scRNAseq

tangming2005's tweet image. New blog post! How to make a multi-group dotplot for single-cell RNAseq data from scratch buff.ly/3SmHeEm #rstats #scRNAseq

TemporalVAE: atlas-assisted temporal mapping of time-series single-cell transcriptomes during embryogenesis. #SingleCell #scRNAseq #TimeSeries #Embryogenesis #DeepLearning #Genomics #Bioinformatics @NatureCellBio nature.com/articles/s4155…

jsantoyo's tweet image. TemporalVAE: atlas-assisted temporal mapping of time-series single-cell transcriptomes during embryogenesis. #SingleCell #scRNAseq #TimeSeries #Embryogenesis #DeepLearning #Genomics #Bioinformatics @NatureCellBio  
nature.com/articles/s4155…

Discover the drivers of gene regulation in your scRNA-seq data with Velorama. Exploiting RNA velocity, Velorama infers causal links between transcription factors and target genes, even in complex branching trajectories. 🌿 Now out in @CellSystemsCP 📜 #scRNAseq #GeneRegulation

rohitsingh8080's tweet image. Discover the drivers of gene regulation in your scRNA-seq data with Velorama.

Exploiting RNA velocity, Velorama infers causal links between transcription factors and target genes, even in complex branching trajectories. 🌿

Now out in @CellSystemsCP 📜 #scRNAseq #GeneRegulation

Our newest preprint is out! We introduce Forseti, a model to predict the splicing status of individual fragments in tagged-end #scRNAseq experiments (biorxiv.org/content/10.110…). 1/

nomad421's tweet image. Our newest preprint is out! We introduce Forseti, a model to predict the splicing status of individual fragments in tagged-end #scRNAseq experiments (biorxiv.org/content/10.110…). 1/

Our paper is out! Want to know more about human and mouse astroglial toxicity in FTLD-GRN thalamo-cortical circuit? jci.org/articles/view/… #scRNAseq #Organoids Fantastic collabs with @Kriegsteinlab @UllianLab @immunox and support from @AFARorg @_BrightFocus @BluefieldProj

EliseMarsan's tweet image. Our paper is out! Want to know more about human and mouse astroglial toxicity in FTLD-GRN thalamo-cortical circuit? jci.org/articles/view/… #scRNAseq #Organoids Fantastic collabs with @Kriegsteinlab @UllianLab @immunox and support from @AFARorg @_BrightFocus @BluefieldProj

Great 1st day of #scRNAseq course at @institut_curie. Many thanks to @jimena75656604 and the bioinformatics team of @ImmunCancer_IC! 🖥️🧬

dario_anobile's tweet image. Great 1st day of #scRNAseq course at @institut_curie.
Many thanks to @jimena75656604 and the bioinformatics team of @ImmunCancer_IC! 🖥️🧬

Scnanoseq: an nf-core pipeline for oxford nanopore single-cell RNA-sequencing. #scRNAseq #LongRead #Sequencing #Bioinformatics #Genomics academic.oup.com/bioinformatics…

jsantoyo's tweet image. Scnanoseq: an nf-core pipeline for oxford nanopore single-cell RNA-sequencing. #scRNAseq #LongRead #Sequencing #Bioinformatics #Genomics
academic.oup.com/bioinformatics…

📢 We are looking for a motivated candidate for a fully funded #PhD to analyze the #transcriptome of polyploid cells from tomato fruit 🍅 at the single cell level #scRNAseq @INRAE_BFP @nathy_go_7 Info here 👇 jobs.inrae.fr/ot-22230

PgpMartin's tweet image. 📢 We are looking for a motivated candidate for a fully funded #PhD to analyze the #transcriptome of polyploid cells from tomato fruit 🍅 at the single cell level #scRNAseq 
@INRAE_BFP  @nathy_go_7 
Info here 👇
jobs.inrae.fr/ot-22230

#AI methods for #denoising of #scRNAseq data have recently raised huge attention Yet, existing approaches lead to dramatically different (dangerous?) data transformations In the most extensive review to date we compare 19 methods and provide guidelines doi.org/10.1093/bib/bb…

AGraudenzi's tweet image. #AI methods for #denoising of #scRNAseq data have recently raised huge attention
Yet, existing approaches lead to dramatically different (dangerous?) data transformations

In the most extensive review to date we compare 19 methods and provide guidelines
doi.org/10.1093/bib/bb…

Had a great time giving my education talk on decoding preclinical models of neuropathies using #scRNAseq 🧬 🧫🔬 @PNSociety1 Really appreciated the insightful questions and engaging discussion!

EidSteph's tweet image. Had a great time giving my education talk on decoding preclinical models of neuropathies using #scRNAseq 🧬 🧫🔬 @PNSociety1 

Really appreciated the insightful questions and engaging discussion!
EidSteph's tweet image. Had a great time giving my education talk on decoding preclinical models of neuropathies using #scRNAseq 🧬 🧫🔬 @PNSociety1 

Really appreciated the insightful questions and engaging discussion!

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