ShihLab
@LabShih
Twitter of the Lab of Dr. Patrick Shih at UC Berkeley
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Active learning enables discovery of transcriptional activators across fungal evolutionary space biorxiv.org/content/10.110… #biorxiv_genomic
Big news from Demirer lab: 4 research papers preprinted! Feedback welcomed: eCIS for plant protein delivery: tinyurl.com/mrx4d878 R2 for targeted insertion: tinyurl.com/5n73za6h Transient expression: tinyurl.com/38p2ud6w Chemotaxis assay: tinyurl.com/3nw9haes 👀⬇️
A truly enabling new approach by Tara Lowensohn, @WillCody_12 and @Sattely_lab to probe plant genetics at scale. Single-gene-per-cell delivery coupled to an effective transcriptional selection system. Libraries in plants. biorxiv.org/content/10.110…
Check out our new preprint! We examined sources of variability of transgene expression in N. benthamiana agroinfiltration and ways to minimize it. Graduate student Sophia Tang observed FP expression in >1,800 plants over the course of nearly 3 years! biorxiv.org/content/10.110…
Causes and consequences of experimental variation in Nicotiana benthamiana transient expression biorxiv.org/content/10.110… #biorxiv_plants
Today the Shih Lab (@LabShih) presents ENTRAP-seq, a high-throughput assay for functional screening directly in plant cells.
We're excited to share another work from our lab this week. We present ENTRAP-seq, the first massively parallel reporter assay to characterize protein-coding libraries in plants. Congrats to Simon, Lucas, and all other co-authors. biorxiv.org/content/10.110…
New OA Article: "Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism" rdcu.be/elJ5z News & Views: "Cooperation and antagonism in Agrobacterium-mediated transformation" rdcu.be/elJ5F
In @ScienceAdvances, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains science.org/doi/10.1126/sc… (1/n)
I am pleased to announce new research from the Shih Lab (@LabShih) published today in Nature Biotechnology on improving Agrobacterium-mediated transformation via binary vector copy number engineering (doi.org/10.1038/s41587…). 🧵A summary thread below🌿
Binary vector copy number engineering improves Agrobacterium-mediated transformation go.nature.com/3CdewQS
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