GeoffTHowe's profile picture. Postdoc @ Aguzzi Lab University of Zurich |SynBio PhD @ UCL | Virology, Gene Therapy, Functional Genetics

Geoff Howe

@GeoffTHowe

Postdoc @ Aguzzi Lab University of Zurich |SynBio PhD @ UCL | Virology, Gene Therapy, Functional Genetics

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Delighted to have successfully defended my PhD last month! We built DNA clearing HEK cells and designed AAV protein variants to study their function and improve AAV manufacturing! Huge thanks to my PI @GenePlumber, everyone @UCLBiochemEng1, examiners @alanlparker & @pauldalby!

GeoffTHowe's tweet image. Delighted to have successfully defended my PhD last month! 

We built DNA clearing HEK cells and designed AAV protein variants to study their function and improve AAV manufacturing!

Huge thanks to my PI @GenePlumber, everyone @UCLBiochemEng1, examiners @alanlparker & @pauldalby!

A grey day in Hamburg spent discussing all things virology and CRISPR screening at the DEFENDER symposium - an EU funded consortium on a mission to identify new targets and develop new therapies against high priority viruses.

GeoffTHowe's tweet image. A grey day in Hamburg spent discussing all things virology and CRISPR screening at the DEFENDER symposium - an EU funded consortium on a mission to identify new targets and develop new therapies against high priority viruses.

Geoff Howe reposted

Repeated head trauma causes neuron loss and inflammation in young athletes: nature.com/articles/s4158…


Geoff Howe reposted

🔬 Funded PhD 'Engineering self-regulating CAR-T cells' #SynBio #EngBio #iGEM2025 Work across mammalian synthetic biology & mathematical modelling. ⏰ Apply by 1pm UK, 5 Jan 2026 Project details: ucl-epsrc-dtp.github.io/2026-27-projec… Project application portal: ucl.ac.uk/epsrc-doctoral…

GenePlumber's tweet image. 🔬 Funded PhD 'Engineering self-regulating CAR-T cells'  #SynBio #EngBio #iGEM2025 Work across mammalian synthetic biology & mathematical modelling. ⏰ Apply by 1pm UK, 5 Jan 2026 Project details: ucl-epsrc-dtp.github.io/2026-27-projec… Project application portal: ucl.ac.uk/epsrc-doctoral…

Geoff Howe reposted

New AAV capsid filling paper! @Cytiva EngD student Qiantong Chen suspected transfected gene expression misalignment in standard procedures, trialled new timings & achieved 7.5-fold more capsid filling #EngBio #AAV cell.com/molecular-ther…

GenePlumber's tweet image. New AAV capsid filling paper! @Cytiva EngD student Qiantong Chen suspected transfected gene expression misalignment in standard procedures, trialled new timings & achieved 7.5-fold more capsid filling #EngBio #AAV cell.com/molecular-ther…

Geoff Howe reposted

A legend is gone. Discovered Type II restriction enzymes (70s), sequenced the first bacterial genome (90s) and synthesised the first bacterial genome (00s).

Prof. Hamilton Smith at J. Craig Venter Institute, a Nobel laureate for discovering type II restriction enzymes, passed away last night. A huge loss in Science, but his legacy will be remembered. @JCVenterInst @NobelPrize jcvi.org/about/hamilton…

Moon_Synth_Bio's tweet image. Prof. Hamilton Smith at J. Craig Venter Institute, a Nobel laureate for discovering type II restriction enzymes, passed away last night.

A huge loss in Science, but his legacy will be remembered.
@JCVenterInst @NobelPrize 

jcvi.org/about/hamilton…


Geoff Howe reposted

Excited to announce PhD position available at the interface of biology/chemistry and mechanical engineering . We are looking to use protein nanowires to make biocatalysis even more sustainable! The position will be at the interdisciplinary MFL UCL East. ucl-epsrc-dtp.github.io/2026-27-projec…


Geoff Howe reposted

Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. doi.org/10.1101/2025.1…

stephentang23's tweet image. Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?

Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.

doi.org/10.1101/2025.1…

Geoff Howe reposted

Vroomm!! Postdoc extraordinaire @GeoffTHowe has used our "T.gonfio" arrayed CRISPRa library to test ALL human GPCRs (of which there are ~860) for cAMP responses to the prion protein, and found many surprising hits. I could tell you their names, but then I'd have to kill you.

AdrianoAguzzi's tweet image. Vroomm!! Postdoc extraordinaire @GeoffTHowe has used our "T.gonfio" arrayed CRISPRa library to test ALL human GPCRs (of which there are ~860) for cAMP responses to the prion protein, and found many surprising hits. I could tell you their names, but then I'd have to kill you.

Geoff Howe reposted

In case you need the world's most powerful CRISPR ablation/activation/silencing reagents, our quadruple-guide vectors are now available at AddGene! addgene.org/Adriano_Aguzzi/


Geoff Howe reposted

CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes: nature.com/articles/s4159…


Geoff Howe reposted

In @NatRevGenet we outline strategies for in vivo pooled (single cell) CRISPR screens for genotype → phenotype mapping in animal models. This contains over a decade of hard earned knowledge by us and the field. Kudos to @AntonioSantinha & Alessio Strano. rdcu.be/eykft


Geoff Howe reposted

I am super excited to announce that I will be starting my lab at the Department of Pharmacology of the University of Zurich in Switzerland next year!

MartinPacesa's tweet image. I am super excited to announce that I will be starting my lab at the Department of Pharmacology of the University of Zurich in Switzerland next year!

Geoff Howe reposted

This is a major success story in today's Science ALSP is a rare genetic condition – life expectancy is about 8 years after symptoms start, and there's no cure But a bone marrow transplant – in 8 patients – halted disease progression, maintained cognition and ability to walk

DrSamuelBHume's tweet image. This is a major success story in today's Science

ALSP is a rare genetic condition – life expectancy is about 8 years after symptoms start, and there's no cure

But a bone marrow transplant – in 8 patients – halted disease progression, maintained cognition and ability to walk

Geoff Howe reposted

🧬Motifs are the new BLAST Folddisco scans 53M AlphaFold structures for catalytic, binding or allosteric patterns in seconds🔎 18× quicker, 3.5× smaller, hits in seconds🌌

AllThingsApx's tweet image. 🧬Motifs are the new BLAST

Folddisco scans 53M AlphaFold structures for catalytic, binding or allosteric patterns in seconds🔎

18× quicker, 3.5× smaller, hits in seconds🌌

Geoff Howe reposted

CRISPR just scored its biggest win yet against Huntington's. The secret? A delivery system called RIDE that sneaks into neurons, makes its edit, then vanishes in 72 hours. Here's what happened 🧵👇

DrDominicNg's tweet image. CRISPR just scored its biggest win yet against Huntington's.

The secret? A delivery system called RIDE that sneaks into neurons, makes its edit, then vanishes in 72 hours.

Here's what happened 🧵👇

Geoff Howe reposted

This is wild! Engineering E. coli bacteria to turn plastic waste into paracetamol (Tylenol) @NatureChemistry nature.com/articles/s4155… nature.com/articles/s4155…

EricTopol's tweet image. This is wild! 
Engineering E. coli bacteria to turn plastic waste into paracetamol (Tylenol) 
@NatureChemistry 
nature.com/articles/s4155…
nature.com/articles/s4155…

Geoff Howe reposted

Published today in @Nature, we describe an approach for single-molecule protein reading on @nanopore arrays. By utilizing ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieved single-amino-acid sensitivity. nature.com/articles/s4158…


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