Katie Galloway
@GallowayLabMIT
Assistant Prof @MITChemE ; mom^4 + wife; enjoys building cell-fate circuits, exploring dna topology, reprogramming the living world, and soccer; soli gloria deo
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🤔How do you design a promoter for stable titration of expression? 💡Try programmable promoter editing with DIAL! 📰Now published at @NatureBiotech 🧵 [1/n] 🔗 below
![GallowayLabMIT's tweet image. 🤔How do you design a promoter for stable titration of expression?
💡Try programmable promoter editing with DIAL!
📰Now published at @NatureBiotech 🧵 [1/n]
🔗 below](https://pbs.twimg.com/media/G4GwsleWgAAzh4G.png)
"By inserting a theophylline-responsive riboswitch upstream of essential genes in Escherichia coli, we created strains whose viability depends on the presence of theophylline. We systematically applied this approach to 29 essential genes, obtained ligand-dependent growth…
A Modular Toolkit For Theophylline-Dependent Synthetic Auxotrophs Via Riboswitch-Gated Essential Genes biorxiv.org/content/10.110… #biorxiv_synbio
I love the elegance of this approach so much! When Michael showed me this data, my jaw dropped. The color streaks provide such a beautiful picture of how genetically distinct strains emerge. Congrats, @baym and team!
Hot off the press! Our latest paper led by @fernpizza , understanding how plasmids evolve inside individual cells. These small pieces of self-replicating DNA live inside bacteria and can transfer antibiotic resistance genes, but they also compete with one another to replicate. 1/
Delighted to finally travel to Australia! A journey almost 20 years in the making. If you're at the UNSW Sydney on November 18th, come hear that story!
Enjoyed the tweetorial? Want to hear more? Come hear @emmapeterman_ live at AIChE this coming Thursday. Details 👇 Thursday, November 6th, 2025 Session #654: Cellular, Protein, & RNA Systems Engineering 8:00 AM, Hynes Convention Center, 310
So you want to change transgene expression: just change your promoter, right? Changing the promoter increases RNA and thus protein levels. What more could be happening? [1/n] Well, promoters don’t just set RNA levels; they uniquely transform how RNAs are transmitted into protein…
![GallowayLabMIT's tweet image. So you want to change transgene expression: just change your promoter, right? Changing the promoter increases RNA and thus protein levels. What more could be happening?
[1/n] Well, promoters don’t just set RNA levels; they uniquely transform how RNAs are transmitted into protein…](https://pbs.twimg.com/media/GuVwps3WEAAyqss.png)
Enjoyed the tweetorial? Want to hear more? Come hear @science_sneha live at AIChE this coming Wednesday. Details 👇 Wednesday, November 5th, 2025 Session #543: Genomic & Genetic Engineering Technologies 2:00 PM, Hynes Convention Center, 310
🤔How do you design a promoter for stable titration of expression? 💡Try programmable promoter editing with DIAL! 📰Now published at @NatureBiotech 🧵 [1/n] 🔗 below
BE Seminar Series this Thurs w @BugajLab, @pennbioeng “Using Light, Temperature, and Protein Condensation to Understand and Control Cells” be.mit.edu/our-community/…
Come see @SuYapeng at #AICHE2025 future faculty poster session! 👇
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